We provide two primary services for our customers:
1) Exosome isolation services — allows any researcher the option to send their biological samples to Clara Biotech for isolation with the ExoRelease™ Isolation Platform
2) Characterization services (includes characterization of surface marker identification, size distribution, concentration measurements, and more).
For additional information on exosomes characterization and Clara’s wet lab capabilities, visit our services page or reach out via our contact page.
The ExoRelease™ Isolation Platform utilizes an unique technology to capture and release exosomes, allowing researchers to explore the possibilities of pure and biologically active exosomes. ExoRelease™ works by using immunoaffinity magnetic bead technology, monoclonal antibodies (mAb) attached to magnetic beads, to capture exosomes. Then the exosomes are released from the magnetic beads using the ExoRelease™ Photo-Release Tool and its photo-release technology.
For additional information, please visit our Science Page.
The ExoRelease™ Isolation Platform is the most capable and innovative exosome isolation solution currently on the market. What sets ExoRelease™ apart is the ability to safely release fully functional exosomes and our proprietary bead technology, which employs unique surface characteristics to enable superior exosome capture.
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ExoRelease™ Product FAQs
We use mouse anti-human IgG Ab's CD9, CD63, and CD81 for our ExoRelease Human Pan Reagent. We can also use your preferred antibodies to customize our ExoRelease Choice Reagent specifically for your needs.
The ExoRelease™ Isolation Platform uses a proprietary photo release process that safely detaches the captured exosomes from the bead.
ExoRelease™ Exosome Characterization FAQs
Currently, there are no limits when it comes to downstream applications. We have helped researchers isolate exosomes for use in analyses involving DNA, RNA, and protein, and have supported a variety of exosome research applications that range from therapeutics to diagnostics.
We evaluated the influence of photo release exposure to exosome molecular contents, including exosomal proteins, DNAs, and RNAs, as shown in the figure below. Based on quality assessment using gel electrophoresis, there was no detectable change observed with or without UV treatment in terms of exosomal protein, DNA, and RNA contents.
This result indicates that there are no negative effects of the light release exposure on exosome molecular contents under our optimized treatment and supports the photo-release of exosomes to provide exosomes with high integrity.
ExoRelease™ Capabilities FAQs
When isolating EVs using alternative isolation technologies like ultracentrifugation (UC) or size exclusion chromatography (SEC), the end sample contains a variety of extracellular vesicles. These various extracellular vesicles could range from smaller vesicles like microsomes to larger membrane-bound subcellular structures like apoptotic bodies, exosome-like vesicles, membrane particles, and ectosomes.
Many researchers use vesicle size as a criteria to differentiate between these various kinds of EVs. However, at Clara Biotech we believe size alone is not a critical criteria as many of the size ranges of these other EVs overlap with the size range commonly used to define exosomes.
Our technology utilizes a combination of exosome-specific characteristics to precisely target exosomes and minimize false positives and contaminants from other non-exosome EVs. These characteristics include vesicle size, molecular content, and a combination of membranous surface markers.
ExoRelease™ is able to isolate exosome subtypes by targeting the surface marker proteins on the exosome. This solution is believed to be superior method to classify and characterize exosomes because of the heterogeneity problem found throughout the exosome industry.
To learn more about how you can isolate specific types of exosome subpopulation, contact us.